Abstract:
This study was conducted to examine the clinical mastitis, to isolate and identify
the bacteria causing the disease and to determine the sensitivity pattern of isolated
bacteria to commonly used antibiotic in Eldamazine locality - Blue Nile State,
Sudan from June to November 2020. A total of 45 milk samples were collected
from dairy cows clinically infected with mastitis and transported to Microbiology
Laboratory in Eldamazine town for bacteriological examination. All samples were
cultured in blood agar, MacConkey agar and purified in Nutrient agar. Gram stain
was used for identification of morphological characteristics of bacteria. Antibiotics
sensitivity test to gentamycin, ampicillin, vancomycin, ciprofloxacin, oflaxacin,
tetracycline and erythromycin. Biochemical tests were done to all isolates. Three
types of inflammation were detected: acute mastitis with high prevalence (62.2%)
followed by chronic mastitis (35.6%) and gangrenous mastitis (2.2%). The isolates
were: 73.4 % Staphylococcus spp, 4.4% Streptococcus spp, 8.9% Bacillus spp ,
4.4% Pseudomonas spp and 8.9% Escherichia spp. The results revealed that the
most sensitive antibiotics on isolated bacteria were gentamycin, vancomycin,
ciprofloxacin, oflaxacin and tetracycline, while resistance was ampicillin and
erythromycin. Pseudomonas spp was resistant to all antibiotics used. In conclusion
mastitis is associated with huge economic loss in the study area.
