Bacteriological Study on Mastitis in Dairy Cattle -Eldamazine Locality – Blue Nile State, Sudan

Abstract

This study was conducted to examine the clinical mastitis, to isolate and identify the bacteria causing the disease and to determine the sensitivity pattern of isolated bacteria to commonly used antibiotic in Eldamazine locality - Blue Nile State, Sudan from June to November 2020. A total of 45 milk samples were collected from dairy cows clinically infected with mastitis and transported to Microbiology Laboratory in Eldamazine town for bacteriological examination. All samples were cultured in blood agar, MacConkey agar and purified in Nutrient agar. Gram stain was used for identification of morphological characteristics of bacteria. Antibiotics sensitivity test to gentamycin, ampicillin, vancomycin, ciprofloxacin, oflaxacin, tetracycline and erythromycin. Biochemical tests were done to all isolates. Three types of inflammation were detected: acute mastitis with high prevalence (62.2%) followed by chronic mastitis (35.6%) and gangrenous mastitis (2.2%). The isolates were: 73.4 % Staphylococcus spp, 4.4% Streptococcus spp, 8.9% Bacillus spp , 4.4% Pseudomonas spp and 8.9% Escherichia spp. The results revealed that the most sensitive antibiotics on isolated bacteria were gentamycin, vancomycin, ciprofloxacin, oflaxacin and tetracycline, while resistance was ampicillin and erythromycin. Pseudomonas spp was resistant to all antibiotics used. In conclusion mastitis is associated with huge economic loss in the study area.