Abstract:
Salbutamol is apharmaceutical drug for the relief of bronchospasm in condition such as asthma.It is sold as racemic mixture in its forms (S)and(R).In this present work 1.3g from sample was crushed and dissolved in amixture of ammonium acetate dissolved in filter distilled water,2-propanol then adjusted PH of 4.5 by adding glacial acetic acid then shaked for 90minute and separation occurred using automatic HPLC.
Identifications of salbutamol were carried out using IR and UV spectroscopy using twobuffers of solutions, phosphate buffer, sodium hydroxide buffer.
In UV data the real λ max were appeared in PH 9and10 due to the amino group and phenolic group respectively.
