Abstract:
Abstract
The study was conducted to investigate the occurrence of
bovine mastitis in HilatKuku,Khartoum, to isolate the bacteria
responsible for causing the disease and to determine the
antimicrobial susceptibility to the related bacteria. Fifty samples
of mastitis milk from fifty dairy cows were collected and
transported to Microbiology Laboratory of The College of
Veterinary Medicine – Sudan University of Science Technology.
Isolation and identification of the bacteria from the collected
mastitic milk was undertaken. The results revealed high
prevalence of Staphylococcus aureus 48% followed by
Streptococcus agalactiae 28%, Proteus spp. 12%,
Starphylococcus hyicus 8% and Streptococcus dysgalactiae 4%.
Three types of inflammation were detected, acute mastitis with
high prevalence (66%) followed by chronic mastitis (32%) and
gangrenous mastitis (2%). Staphylococcus aureus was found to
infect one quarter causing chronic mastitis and sometimes
acute mastitis. Starphylococcus hyicus was found to infect one
quarter causing chronic and acute mastitis. Streptococcus
agalactiae was found to infect one quarter causing acute and
chronic mastitis. Streptococcus dysgalactiae was found to
infect more than two quarters causing acute mastitis. While
Proteus spp. involved the four quarters causing mainly acute
mastitis. The effective antibiotics used during this study against
most of the isolated bacteria were Gentamycin, Amikacin,
Chloramphincol, Oflaxacin and Ciprofloxacin. Some isolates
showed resistant to some antibiotics which were Penicillin,
Ampicillin, Cefotaxime, Cloxacillin and Gatifloxacin.
Histopathological changes of the examined parenchymal tissue
showed parenchymal damage and formation of abscesses.
Seven samples from the ten examined parenchymal tissues
samples showed necrosis of the mammary gland. These were
mostly caused by Staphylococcus aureus causing chronic
mastitis. Two samples showed hemorrhagic interstitial tissue
mainly caused by E. coli and Streptococcus spp. causing acute
mastitis. Only one sample showed necrotic alveoli in the
.parenchymal tissue due to gangrenous type of inflammation
