Abstract:
The preseut stady was carried out to compare the efficiency of direct
microscopy , in vitro cultivatior and direct agglutination test (DAT)
for diagnosis of visceral leishmaniasis (VL) . this stady was carried
out in omdurwan pediatric hospital in Khartoum state between
March and October 2011 , which included patiens clinically
suspected of VL . The number of suspected VL cases was 50 and
the following was performed ;
- Bone marrow aspirates for direct microscopy and in vitro
cultivation .
- Serum samples for DAT .
The microscopical examination of bone marrow smears revealed the
presence of amastigotes In 10 (20%) cases in vitro cultivation of
bone marrow aspirates give positive growth of L . donovan
promastigotes in 11 (22%) case and negative in 39 (78%) of case
and DAT was positive in 20 (40%) of case and negative 30(60%) of
the results of DAT obtained were compared to the results of direct
mircroscopy and invitro culture of Bone marrow aspirates this was
shown that DAT was more sensitive than microscopy and invitro
culture .