Abstract:
The aim of this research was to explore the singlet oxygen emitted after
irradiation Dibenzocyanine 45, Methylene Blue and Rhodamine 6G dyes after
irradiation using two lasers with different wavelengths, so that the singlet
oxygen can be used in photodynamic therapy (PDT).
Three samples of DDTTC 45 were prepared by dissolved in propanol,
methanol and acetone respectively.
Two samples of Methylene blue prepared by dissolved in methanol and
ethanol. One sample of Rhodamin 6G was dissolved in methanol.
UV visible spectrometer was used to record the absorption spectra for the
solvents and dyes. Two laser sources were used to irradiate the samples.
Firstly diode laser (532nm) with output powers of 100 mW, secondly the
diode laser (671nm) with output power of 100 mW. USP2000 spectrometer
connected to PC, was used to collect and record the emission spectra after
different exposure times.
The results showed that the most efficient sample to produce the singlet
oxygen was the DDTTC 45 dissolved in acetone after irradiation by the diode
laser 671 nm with exposure time of six minutes which indicated a peak of
singlet oxygen at (703 nm). It is worth to mention that, no evidence was
noticed for a singlet oxygen production from the other samples.