Abstract:
The aim of this study was to detect extended spectrum ß-lactamase
(ESBL) in Klebsiella pneumoniae isolated from sputum. A total of 100
sputum specimens were collected from Alshaab Teaching Hospital during
April to June 2012. Bacteriological tests of sputum specimens were
performed
for
Klebsiella
pneumoniae
including
inoculation
on
MacConkey’s agar and blood agar. The identity of the isolates was
confirmed by biochemical tests. Out of 100 sputum specimens; 21 (21%)
Klebsiella pneumoniae was recovered from patients with different age
groups.
Susceptibility test and extended spectrum ß-lactamase
production were carried out for each isolate. The isolates were resistance
to nitrofurantoin, naldixic acid and co-trimoxazole and sensitive to
imipenem. Screening tests for ESBL were performed against four types
of third generation cephalosporin (3GC) and amoxyclave. Out of 21
isolates, 19 were sensitive and two were resistance. Double disc synergy
test was done for the two isolates that were found resistance to the 3GC
to detect ESBL an the result was positive .This study showed that
Klebsiella pneumoniae considered to be one of ESBL producer.
