Abstract:
This study was carried out in Khartoum state during the period between May 2007 to
March 2008 to detect Providencia rettgeri in patients with urinary tract infection. Forty-
six specimens of urine were collected from different hospitals in Khartoum state.
Bacterial DNA was extracted from each urine specimen by using phenol chloroform
method, and then the real time PCR technique was adopted to detect P. rettgeri in these
specimens. This study showed that the positive results were 4.3% and the negative
results were 95.7%. The study concluded that the real-time PCR is a powerful technique
for fast identification of Providencia rettgeri from urine specimens compared to the
bacteriological culture and it has the potential to be used in a diagnostic microbiology
.laboratory