Abstract:
The design and implementation of a laser system used in clinical
analysis to measure some human blood components is achieved in this
work.
A measurement for determining the absorption spectrum of the
standard samples of the glucose, cholesterol, total protein, albumin and uric
acid is done using the single beam spectrophotometer, and the laser
spectrophotometry techniques. Absorption peak in the range (505-550 nm)
are noticed,
according to the results of these mseasurements, which are
in good agreement with the spectrum results, a laser system consist of a
frequency doubled diode pumped Nd YAG of wavelength 532nm and
1mW power, silicon photo detector, sample cell with holder, 100-105 gain
amplifier connected to a digital multimeter, are utilized to measure the
concentrations of the above mentioned components for ten different
samples.
The obtained results of these ten samples are compared with that
measured by using the spectrophotometer. The comparison is done by
calculating the correlation coefficient, the relative standard deviation and
studying the relationship between the absorbance and the concentration.
The results show that, the suggested laser techniques could be used
with high efficiency, to measure the concentration of the human blood
component, which its absorption peaks within the range of 505-550 nm
wavelength, faster, easier and with low cost compared to traditional
methods, and without no any complications.
