Abstract:
* This study aimed to determine the effect of He-Ne laser
10 mW and 632.8 nm wavelength on bacteria that can
cause Urinary Tract Infection in the presence of the
photosensitizers. Toluidine blue(TBO) and crystal violet
(CV).
* The samples for this study were collected from twenty
patients with UTI in addition to two other
microorganisms (E. coli ATCC25922), S.aureus (ATCC
25923 ) obtained from laboratory collage – university of
Khartoum, as standard organisms .
* The samples were irradiated with different exposure
times and different dye percentages.
* The colonies were counted before and after irradiation
and compared with the number of colonies of the control
groups without irradiation.
* The result showed that there was a moderate reduction
in number of colonies with the increasing of the exposure
time and dye percentage incase ofS. aureus
ATCC25923was reduced from 1440 colonies at zero
timeto880,800 and 600 colonies at 30 (min) with the
percent of TBO 5%,15%and 45% respectively , while in
E .coli ATCC25922 reduced from 1440 colonies at zero
timeto900,800 and 550 colonies at 30 (min) with the
percent of TBO 5%,15%and 45% respectively , in the
isoled organism Pseudomonas aeruginosa was reduced
from 660 colonies at zero timeto540,510 and 480
colonies at 30 (min) with the percent of TBO
5%,15%and 45% respectively ,also the isoled Proteus
mirabilis was reduced from 740 colonies at zero
timeto720,680 and 650 colonies at 30 (min) with the
percent of TBO 5%,15%and 45% respectively ,
* Finally from these results it was noted that the killing
effect of bacteria increased with the increasing of
exposure time using 45% TBO as photosensitizer.
