Abstract:
This study was designed to monitor antibiotic residues in tissues of poultry from Khartoum state. A total of 360 tissue samples, (muscle, liver and kidney) were collected randomly from the market in Omdurman, Khartoum and Khartoum North localities. The samples were taken in summer, autumn and winter seasons (each season 120 samples). All samples were qualitatively screened for antimicrobial inhibition test using Bacillus sibitilues ATCC-6633. To quantify the oxytetracycline positive samples were further analyzed with high performance liquid chromatography (HPLC) after screening the results revealed that 52 (14.4%) samples were positive. Muscle tissues showed high percentage (18.8%) than those liver and kidney (15.0%) each. The positive samples obtained from Omdurman, Khartoum and Khartoum North were 20 (16.6%), 18 (15.0%), 14 (11.6%) respectively. The positive samples in summer were higher (35.8%) than autumn (10.8%) and winter (6.6%).Samples were analyzed using high performance liquid chromatography (HPLC UV/VIS) at 360 nm using isocratic system with mobile phase consist of oxalic acid: acetonitrile: methanol (75:20:5) and reverse phase (C18) column (150mm⨉4.6, 5-μm). The result revealed that 91% of the samples contain oxytetracyclines residues ranged from 3972.78 – 34.58 ppb, with 30% over the MRL. OTC residue presents in all tested liver and kidney sample while 70% of the tested muscle showed positive result. Comparing with the MRL, liver sample showed the highest percentage 54.5% followed by muscle 42.9% and kidney 8.3%. Generally OTC was detected in a high percentage in poultry tissues emerging the need for development of practical and scientifically based monitoring system for detection and quantification of antimicrobials residue all over the country.
