A Comparative Study on three Wound Managements in Donkeys, Gobiesh Locality- Sudan

Abstract

This study was conducted to compare and evaluate the efficacy of different three treatments that used in donkey’s wounds, such as coffee powder and spider web as traditional wound treatments, with Oxytetracyclin powder is a common medical treatment as control group in area of Gobiesh locality, West Kurdufan State, Sudan. The study was carried out in faculty of Veterinary Medicine West Kurdufan University. A total number of clinically sound thirty-six local breed donkeys of both sexes10 female and 26 mail were used in this study with mean ± stander deviation age and body weight 7±4.1 years and 93± 7.8 kg respectively. Animals were kept for adaptation period of 7 days prior to experiment and all groups receivedivermectine best 500 mg as well as anti-tetanus injection, animals were fed on green fodder and hay with free access to water allowed. Animals were divided into three equal groups, each of 12 donkeys. Ten Centimeters length and three centimeters depth of Gullotus area were incised under local anesthesia. Wound left opened and untreated two days’ post incision, the first group treated with coffee powder, second group covered with spider web while the third group (control) dressed using oxytetracyclin powder. General observation of inflammatory sings was studied such as wound bleeding, oedema, congestion, odor, and pus formation two weeks post treatment, clinical parameters such as respiratory rate, heart rate and rectal temperature were recorded as well as hematological parameters (White Blood Cells count) were screened at intervals 0, 2, 4 and day 6. On the other hand, wound healing processes were observed during wound healing such as wound contraction, Scab formation, Scab cracking and detachment of scab formation at the site of wounds. Histopathological evaluation was done in all groups to determine the grade of wound healing., no significant differences were found in clinical and hematological indices among all treated group, however, significant difference (p value ≤ 0.05) in bleeding were observed at day 2 post wounding in spider web treated group (25%), wound oedema revealed no significant changes in all treated group. On the observation of the congestion at the wound site showed a significant change in all treated group at interval 2,4, 6 and 8 days after treatment, although spider web group showed the higher incidence of congestion compare with the other two groups, wound contraction was significantly observed on day 2, 8, 10 post treatment, however coffee powder and spider web treated groups showed the higher percentage of wound contraction occurrence at day 6 (100%). Scab formation was clearly observed excellent at day 4,6, 8, 10days12 showed excellent in group A C bad in group Band post treatment specially in coffee powder group and less in noticed Spider web and tetracycline groups, scab cracking percentage study were significantly noticed on days 4, 6, 8 and 10 following treatment in all donkeys, especially control group that treated using tetracycline powder (day 4 and day 6). Wound scab detachment was observed during two weeks after treatment, the percentage of wound scab detachment were more observed on day 4 and day6 post treatment, however, spider web treated groups showed no wound scab detachment occurrence till the day 10 following wounds management, Histopathological examination that carried out to determine the wound healing grades revealed that in coffee powder 10%, 30% and 60% were in grade 1,2 and grade3 respectively, spider web managed group showed 60%(grade 2) 40% (grade3) and the control group (tetracycline treatment group) 80% and 20% were detected in grade 2 and grade 3 respectively. Finally, it could be concluded that the treatments were found to be safe and reliable in treating superficial wound in donkeys. However; coffee powder was found to be superior to other treatments that clearly observed in terms of short healing time, ascent removal andbleeding suppressed.