Abstract:
In this work Raman and FTIR spectroscopy were used for spectroscopic
characterization of leukemia blood samples. Fifteen samples were
collected from leukemia patient's types:
acute, chronic lymphocytic
leukemia and acute chronic myeloid leukemia. The patients were
diagnosed by histopathologies in Radiation and Isotopes Center Khartoum
(RICK) and Alamal Hospital.
The analysis of the Raman spectra was done for the peaks of proteins:
1240-1310, 1548, 1655 ,3200 ,3700-3500 and 628 cm-1. These bands are
attributed to α-helical of amid I, amid II, amid III, N-H bond of amid A
and amid VI, respectively. Also the bands at 1120 and 835 cm -1 that are
representing the strong C-N vibration bond of ribose and the vibration
bond of CH2. Raman shift was recorded at wavenumber 1140 cm-1 which
represents the stretching vibration of amino acid.
The Raman shifts observed in the wavenumbers 2800, 1446 cm -1 and
2921cm-1 are attributed to lipids and C-H asymmetric vibration in fatty
acid. The Raman shifts observed at 1310 and 1499 cm -1 represent the
nucleic acid, the band at 779cm-1 represent the CH2 group of nucleic acid.
Spectroscopic differences were noticed between normal and cancer
samples using FTIR at (2091, 3450 cm-1) for lipids, (1548, 1650 cm-1) for
proteins and (1170, 1303 cm-1) for nucleic acids.
The intensities of leukemia samples peaks are increased compared with the
intensities of peaks in normal blood samples recorded by FTIR
spectrometer with no difference in position of the absorption lines.
When comparing the two techniques, it can be concluded that the Raman
spectroscopy has higher resolution and is more precise than FTIR
spectroscopy. Both techniques can be used for diagnosis of leukemia blood
samples efficiency.
