Abstract:
This study was aimed to investigate the presence of
Staphylococcus aureus among patients with wound and urinary
tract infections and to subject the isolated S.aureus to
Polymerase Chain Reaction (PCR) in Khartoum state.
The study was performed during the period from March to June
2011. It included 100 samples from wound and urine infection
from patients with different sex and age .The samples were
cultured on Blood agar ,Macckoncy’s and CLED agar incubated
aerobically at 37ْC. Gram stain was done for bacterial isolates.
Biochemical tests were used to identify isolated bacteria.
Polymerase Chain Reaction amplification target gene arcC
(crabmeat Kainase ) was done for isolated S.aureus .The study revealed that S.aureus was isolated from samples was
)23.8% and Methicillin-resistant Staphylococcus aureus(MRSA
. %08 percentage was
DNA extraction By Phenol chloroform isomayle alcohol were
done for 10 isolates ,PCR showed 2 isolates (20%) were arcC
.primer recognized and 8 isolates (80%) were not arcC primer
This study showed that the identification of Staphylococcus
aureus by polymerase chain reaction by amplification arcC gene
is not desirable due to the limited of this gene in the strains of
.S.aureus
