Abstract:
The main aim of this study was to detect M.catarrhalis in children
with otitis media in Khartoum State, during the period from
January to March 2011.
A total of 110 specimens of middle ear discharge were collected
by sterile swabs from Khartoum Center of Ear, Nose and Throat
(ENT), Head and Neck Surgery, the specimens were then cultured
on chocolate agar, sheep blood agar and MacConkey agar, in
which 90 gave growth (82%) while 20 gave no growth (18%).
There were 3 M.catarrhalis (3.3%) isolated from middle ear
discharge of infected children. The colonial morphology, Gram
stain, biochemical tests and susceptibility tests of the isolated
M.catarrhalis were compared with the reference strains (ATCC
23246, ATCC 25240 and ATCC 25238).
The isolated M.catarrhalis colonies on chocolate agar and sheep
blood agar could be pushed along the surface of the agar like a
hockey puck. Also it was positive for oxidase, catalase, DNase,
tributyrin tests and reduced nitrate to nitrite.
%4.2 The percentage of M.catarrhalis among males was 4.2% and
.among female patients
When susceptibility test was done, all isolated M.catarrhalis were
,found to be sensitive to Amoxyclav, azithromycin, ceftazidime
,chloramphenicol
,cephotaxime
,cephalexin
,ceftriaxon
ciprofloxacin, co- trimoxazole and erythromycin. Whereas all of
them were resistant to ampicillin. Moreover M.catarrhalis isolates
.(100%) produced β-lactamase enzyme
