Abstract:
Human papillomaviruses (HPV) have great public role in the
development of cervical carcinoma causing fatal consequences.
The aim of this study was to detect the presence of HPV-18 virus
by amplification of the E6 gene by real time-polymerase chain
reaction (rt-PCR) analysis utilizing the screening SYBER green.
A total of 50 cervical cancer biopsy samples (parrafin embedded)
of patients attending Khartoum Teaching Hospital, National
Tropical and Health Laboratory, Army Medical Hospital and Soba
.countries
University Hospital were collected to be processed for further
study. This study was conducted during the period from April to
September 2012.
The specimens were treated to remove paraffin, and DNA
extracted. The E6 gene of HPV-18 encoding the oncoprotein
product was amplified by the rt-PCR assay using the SYBER green.
Out of the total 50, 18 (36%) specimens yielded positive results;
resembling the sigmoid curve of the positive control, while 32
(64%) were negative, in addition most of the positive results were
concentrated among age group (29-50 years) with frequency rate
12 (24%).
In conclusion, this study showed that HPV-18 has great role in
initiating cervical cancer with no noticeable variation on the
occurrence of the virus in comparison with local and global
studies in Sudan and other countries.
Specimens
were
treated,
paraffin
removed
and
DNA
was
extracted and then E6 gene of HPV-18 was detected by real-time
PCR.
Out of the total, 18 (36%) of specimens showed positive results
while 32 (64%) were negative and most of the positive results
were
concentrated
among
age
group
(29-50
years)
with
frequency rate 12 (24%).
In conclusion, this study found there is a role of HPV-18 in cervical
cancer with no greater variation in the frequency of this virus as
comparing with results of other studies done in Sudan and other countries
