Abstract:
In this study the fixed oil of the medicinally important species- Brassica nigra was investigated. Phytochemical screening revealed the presence of flavonoids, tannins, alkaloids and saponins. The antimicrobial potential was measured via the cup plate agar diffusion assay against six standard human pathogens and significant results were obtained .
The oil was analyzed by GC-MS where 31 constituents were detected being dominated by: 13-docosenoic acid methyl ester(33.53%),9,12-Z,Z-octadecadienoic acid methyl ester(16.20%),11-eicosenoic acid methyl ester(12.12%),9,12,15-octadecatrienoic acid, methyl ester (10.75%), 9-Z--octadecenoic acid methyl ester(5.90%) and hexadecanoic acid methyl ester( 4.26%).