Abstract:
In this study fixed oil from Spinacia oleracea seed was extracted using hexane as solvent, the percentage of the oil was(4.7%). The oil was studied by GC-MS . 24 components were identified, the main components were: Linoleic acid (38.5%) Oleic acid ( 24.9%), and Palmitic acid ( 16.7%).
Some physicochemical properties were measured : peroxide number ( 10.4), acid value ( 22.1), density (0.93), refractive index (1.48). The oil was screened for antimicrobial activity against six standard human pathogens and promising results were obtained.