Abstract:
Carbapenems are one of the most powerful B-lactam antibiotics against bacteria
especially multidrug resistant isolates. This is a laboratory-based study conducted to
detect carbapenem-resistant Klebsiella pneumoniae (K. pneumoniae) isolated from
patients with different diseases.
A total of sixty (n=60) Klebsiella isolates were obtained from Microbiology Laboratory,
Tawam Hospital, UAE. The isolates were recovered from both males and females with
different age and nationality. All of the isolates were checked for purity by streaking on
nutrient agar, then examined microscopically. Gram stain and Vitek2 was used to confirm
the identity of the isolates. Antibiotic susceptibility tests were carried out using Kirby
Bauer technique and confirmed by Vitek2 machine. Carbapenem-resistant Klebsiella
pneumoniae was detected by Modified Hodge Test.
Re-identification of the isolates confirmed that all isolates (n=60) were K. pneumoniae.
Study on antibiotic susceptibility of the isolates revealed that the resistant was 100% to
Ampicllin, Ceftaxidime, Cefruxime/Axetil, Cephalothin, Aztroenam, and Ertapenem. The
resistant to the rest of the antibiotics range from 3.3-98.3 (Table 2)
The result revealed that all isolates of K. pneumoniae were carbapemen-resistat. The
study concluded that K. pneumoniae were highly carbapemease produce. Deriving
policies for treatment plan nationwide to control KPCs and strict hospital monitoring,
sterilization and intervention plans are highly recommended. Further studies with large
number of isolates are required to validate the results of this study.
