Abstract:
This is a case control study conducted during March to April 2015, at Omdurman locality in Military Hospital Obstetric Unit, to measure the complete blood cells count in Sudanese pregnant women at third trimester of pregnancy. Eighty pregnant women at the third trimester of pregnancy and Forty apparently healthy non pregnant women with matched ages were selected as control group. All participants were informed about research before collection of samples and informed consent was obtained from each. A questionnaire was designed to collect information about the study group as age, number of children, month of pregnancy, history and number of abortion, interpregnancy interval and supplementation intake. 2.5 ml venous blood was collected from each participant in k2EDTA anticoagulant container. Automated hematological analyzer (Sysmex KX21N) was used to measure Complete Blood Count, and the results were analyzed by independent T test and one way ANOVA test of the SPSS computer programme.
The results of this study showed significant decrease in Hb (11.5±2.0 g/dl versus 12.2±1.1 g/dl), PCV (36.1±3.2% versus 37.9±3.4 %), RBCs count (4.1±.4×1012/L versus 4.7±1.2 ×1012/L), platelets count (237.9±59.8×109/L versus 276.3±61.1×109/L), lymphocytes percentage (24.2±9.4% versus 29.2±9.1%) of pregnant women compared to control group respectively. Signifcant increase in WBCs count (7.8±2.4×109/L versus 6.8±2.1×109/L), percentage of neutrophil (63.1±10.3% versus 59.2±16.1%), Absolute Neutrophil Count (5.3±2.7×109/L versus 4.3±1.9×109/L), Platelet Distribution Width (14.9±2.19 versus 12.8±2.4), Red cell Distribution Width - coefficient of variation (14.6±1.8% versus 14.0±1.0%) of pregnant women compared to control group respectively. No significance differences in, Percentage of Absolute Lymphocyte Count, mixd cells, Mean Platelet Volume, Mean Cell Volume, Mean Cell Hemoglobin and Mean Cell Hemoglobin Concentration in test group compared with control group.
The study showed a significant decrease in Hb related to presence of history of abortion and significant decreased in Hb, MCV and MCH by increased the frequency of abortion. A significant decrease in Hb and PCV related to interpregnany interval and Irregular supplementation intake like iron and folic acid was observed and no significant variations in Hb level, PCV and RBCs count related to age groups and number of children.
The study was concluded: there was significant decrease in Hb, PCV, RBCs count, lymphocytes percentage and platelets count of pregnant women compared with control (p< 0.05), significant increased in means of TWBCs and neutrophil's percentage and absolute, RDW-CV and PDW of pregnant women more than control (p < 0.05). No significant differences in Absolute Lymphocyte Count, Mean Platelet Volume , MCV, MCH, MCHC and percentage of mixed cells (p> 0.05).
