Effect of Heamolysis on Aspartate Amino Transferase, Potassium and Phosphate Measurement

Abstract

An experimental study conducted during the period October 2008 to March 2009, in ALNAW hospital and KHARTOUM teaching hospital research and laboratory unite. About twenty different pooled plasma were collected from a 80 apparently healthy volunteers, heamolysate was prepared by freezing at -20 over night, serial dilution of haemolysate was prepared (0.125, 0.25, 0.5, 1, 2, 4, 8 g/dl), each pooled plasma was dispense into different 8 tubes (900μl in each tube) and then added 100 μl from different concentration of heamolysate in each tubes except in compare tube (base line) was added 100 μl of distilled water, and then measure plasma AST, potassium and phosphate in each tubes. Plasma level of AST and phosphate were measured using Hitachi 902 Analyzer. Plasma level of potassium was measured using ion selective electrode. The result showed plasma AST, potassium and phosphate were significantly raised at hemoglobin concentration 0.4 and 0.8 g/dl (P<0.01), whereas plasma potassium and AST was significantly increased at hemoglobin concentration 0.1 and 0.2 g/dl (P<0.01), the plasma potassium only was significantly increased at hemoglobin concentration 0.05 g/dl (P<0.05).