الكشف عن المكونات الكيميائية ودراسة الفعالية ضد الميكروبات لجذور نبات الطندب

Abstract

This study is on the root bark of the plant Capparis decidua, which is used in the traditional medicine of Sudan.The root bark was found to contain alkaloids, flavonoids, tritrepenes, and saponins. One of the alkaloid isocodonocarpine was isolated and purified by thin layer chromatography (TLC) on silica gel using solvent system Chloroform : Methanol(5:1) respectivilly, from the ethanolic extract. The chemical structure of this alkaloid (shown below) was determined by comparing its infra- red and ultra- violet spectra with refrence spectra. H HN N H O N O O OH OM e Isocodonocarpin The genreal properties of lipids from hexaneic extract was determined by comparing its U.V. and I.R. spectra with refernce spetra .and it was found to be a surface wax.The antimicrobial activity of the ethanolic extract ,and the isolated pure isocodonocarpine alkaloid exhibited strong antimicrobial activity against gram positive bacteria (Bacillus sublilis)and, (Staphlococcus aureus),and exhibited weak antimicrobial activity against gram negative bateria (Escherichia coli)and, (Pseudoomonas aeruginosa)and their two types of fungi (Candida albicans)and,(Aspergillus niger). Whereas the water extract from the root bark of Capparis decidua was found has no antimicrobial activity against all types of bacteria, and fungi.