Physicochemical Studies of the Hydroxylation Pattern in the Major Flavonoid of Tamrindus Indica

Abstract

Phytochemical screening of the alcoholic extract of the fruits of tamrindus indica indicated the presence of flavonoids . The crude product obtained from the alcoholic extract was analysed by thin-layer chromatography, which indicated the presence of a single flavonoid..This flavonoid was obtained in the pure state by preparative thin- layer chromatography. The behavior of the flavonoid under UV light as well as its colour reactions indicated that it was a dihydrochalcone. The dihydrochalcones was subjected to spectral studies with shift reagent and the compound was found to be dihydrochalchone without any hydroxylation in 3-, 4’-, 5- and 7- OH and it is devoided of catechole.