The use of polymerase chain reaction (PCR) in confirmation of the microbiologically isolated Staphylococcus aureus from wound and urinary tract infections

Abstract

This study was aimed to investigate the presence of Staphylococcus aureus among patients with wound and urinary tract infections and to subject the isolated S.aureus to Polymerase Chain Reaction (PCR) in Khartoum state. The study was performed during the period from March to June 2011. It included 100 samples from wound and urine infection from patients with different sex and age .The samples were cultured on Blood agar ,Macckoncy’s and CLED agar incubated aerobically at 37ْC. Gram stain was done for bacterial isolates. Biochemical tests were used to identify isolated bacteria. Polymerase Chain Reaction amplification target gene arcC (crabmeat Kainase ) was done for isolated S.aureus .‫‪The study revealed that S.aureus was isolated from samples was‬‬ ‫)‪23.8% and Methicillin-resistant Staphylococcus aureus(MRSA‬‬ ‫. %08 ‪percentage was‬‬ ‫‪DNA extraction By Phenol chloroform isomayle alcohol were‬‬ ‫‪done for 10 isolates ,PCR showed 2 isolates (20%) were arcC‬‬ ‫.‪primer recognized and 8 isolates (80%) were not arcC primer‬‬ ‫‪This study showed that the identification of Staphylococcus‬‬ ‫‪aureus by polymerase chain reaction by amplification arcC gene‬‬ ‫‪is not desirable due to the limited of this gene in the strains of‬‬ ‫.‪S.aureus‬‬