Assessment of Ciprofloxacin Resistance among Bacteria Isolated from Patients with Urinary Tract Infection

Abstract

This is a cross-sectional study, carried out during the period from June to September 2012. The objective of this study was to assess ciprofloxacin resistance among bacteria isolated from patients with UTI. 133 Urine specimens were collected from patients suspected of having UTI, attended Omdurman Teaching Hospital. The specimens were cultured on blood agar and CLED agar for primary isolation of the causative agents. The identification of the isolated bacteria was done by colonial morphology, Gram 's stain and biochemical tests. Ciprofloxacin resistance was assessed by modified Kirby-Bauer disk diffusion method. The study results showed that 72 (54.1%) out of 133 specimens yielded bacterial growth, while the rest 61(45.9%) failed to demonstrate any bacterial growth. Among the isolates there were 61 (84.7%) Gram –negative and 11 (15.2%) Gram-positive. Bacteria identified were E. coli 37 (51.4%), Klebsiella pneumoniae 10 (13.8%), Enterococcus faecalis 8 (11.1%), Proteus mirabilis 7 (9.7%), Pseudomonas aeruginosa 4 (5.6%), Staphyllococcus aureus 3 (4.2%) and Klebsiella oxytoca 3 (4.2%). Study on the assessment of ciprofloxacin resistance revealed that the resistance of Klebsiella pnuemoniae was 7 (70%), Enterococcus faecalis 5 (62.5%), E. coli 23 (62.2%), Pseudomonas aeruginosa 2 (50%), and Proteus mirabilis 1 (28.6%). However, there was no resistance Staphylococcus aureus and Klebsiella oxytoca. ‫‪The study concluded that the resistance to ciprofloxacin was high‬‬ ‫‪Klebsiella‬‬ ‫.‪i.e‬‬ ‫‪Pseudomonas‬‬ ‫‪uro-pathogens‬‬ ‫,‪coli‬‬ ‫.‪E‬‬ ‫‪important‬‬ ‫,‪faecalis‬‬ ‫‪most‬‬ ‫‪Enterococcus‬‬ ‫‪the‬‬ ‫‪among‬‬ ‫,‪pnuemoniae‬‬ ‫‪aeruginosa and Proteus mirabilis. Further studies are required to‬‬ ‫.‪validate this result‬‬