Assessment of Resistance in Nosocomial Multi-drug Resistant in Gram- negative Bacteria Isolated from Infected Wounds

Abstract

Nosocomial Multi-drug Resistant Gram-negative bacterial infections are one of serious health problems worldwide. The objective of this study was to isolate, identify and assess nosocomial multi-drug resistant Gram-negative bacteria in infected wounds One hundred clinical swap samples were collected from patients with infected wounds. The identification of the isolated organisms utilized cultural characteristics, Gram’s stain, and biochemical tests. Antimicrobial susceptibility testing was carried out by disk diffusion method on Mueller Hinton agar medium by Kirby Bauer technique. Out of the total number of specimens investigated, 37 of them showed no growth. 38 isolates were Gram positive cocci and 25 isolates were Gram negative bacilli. From the 25 isolates identified as gram negative bacilli, 17 isolates were P. aeruginosa, 4 isolates were E. coli, 2 isolates were K. pneumoniae and 2 isolates were P. vulgaris. 23 isolates were sensitive to all antibiotic disks, while two isolates were multi-drug resistant. The study concluded that P. aeroginosae and E. coli were the most common Gram- negative pathogens isolated from the nosocomial infections in wound infected patients. Further studies are needed to confirm the present results.