Development of Spectrophotometric Method for Determination of Trimethoprim Drug with Mixture of Metol andPotassium hexacyanoferrate (ΙΙΙ) Reagent

Abstract

This research aims to spectrophotometric determination of trimethoprim anti bacterial in urine stream drug in their pure and pharmaceutical forms , this method depend on complex formation between TMP and mixture of metol and potassium hexacyanoferrate (ΙΙΙ) , the complex gave brown color, reaction of the selected drug reagents were carried out in solutions, TMP was dissolve in mixture of methanol and water (1:1) , the complex was measure spectrophotometriclly at UV-Vis , maximum absorbance of the colored complex occurred at λ=420 nm. And also the effect of the parameters ( time , temperature , PH, reagent concentration and volume add) was studied at the λmax of reaction product , the results showed that , the stoichometric ratios is ( 1 : 1 ) . Under optimum conditions the absorbance of the TMP complex where found to increase linearly with increase in concentration of the TMP, statistical calculation of standard deviation ( SD ) , relative standard deviation ( RSD % ) and recovery % prove these results . The proposed method was successfully applied to determine of TMP in pure form and pharmaceutical formulations with good precision and accuracy.