Seroprevalence of Brucellosis among Reproductive Women in Kamleen area in Gazira State

Abstract

This was descriptive cross-sectional study conducted to determine prevalence of human acute brucellosis among females (15-55 years) in Alkamleen area, in Gazeria state, Sudan. In the period from March to July 2015. A total of one hundred and fifty females (n=150) were included in the study. The diagnosis of acute brucellosis was based on slide agglutination test as screening test and confirmed all positive samples by Indirect Enzyme Linked Immunoassorbent Assay for IgM. Out of the blood samples, students were 55 (36.6%),Veterinarians 8 (5.3%), housewives 70 (46.6%), livestock breeders 17 (11.3%) Hundred out of 150 (66.6%) showed positive agglutination in screening test, B.melitensis was found to be major cause of brucellosis All the veterinarians were free from anti-Brucella antibodies in screening test while the live stock breeders showed 7 (41.18%) positive in screening. The students showed 34 (61.8%) positive and the housewives showed 59 (84.3% ) positive Out of 150 cases, 77(51.3% ) was asymptomatic, where 73( 48.6%) showed symptomatic cases. From all symptomatic cases 52 (71.2%) showed positive agglutination and from asymptomatic cases 48 (62.3%) showed positive agglutination. In all symptomatic and asymptomatic cases two samples one for each showed positive reaction when ELISA was used. By indirect ELISA specific for IgM showed two positives, one of them was from housewives category at middle age group (29-41 year) and the other was from livestock breeders at young age group (15-28 year).housewife was symptomatic, where livestock breeder was asymptomatic