Isolation and identification of Staphylococcus species in Haemodialysis Patients in Khartoum State

Abstract

Staphylococcus species are the most important pathogens in hospitalize renal failure patients especially those undergoing haemodialysis. The objective of this study was to isolate and identify the different Staphylococcus species from haemodialysis patients in Khartoum Sate. The study was conducted during the period from February to May 2015. Patients with end-stage renal disease treated with maintenance haemodialysis were enrolled in this study. Pretested structured questionnaire was used for collection of demographic data from each patient. Blood samples were collected from both sexes (55 males and 60 females). The blood samples were inoculated directly in Brain Heart Infusion broth under aseptic condition, and transported directly to the Research Laboratory of Sudan University of Science and Technology for processing. The bacteria were identified by Gram stain and biochemical tests. Out of 115 blood samples investigated, only 33(29%) samples revealed positive bacterial growth in Brain Heart Infusion broth. The identified Staphylococcus species were S. aureus subsp. aureus (3%), S. intermedius (6.1%), S. lutrae (3%), S. epidermatis (3%), S. schlerferi subsp. schlerferi (3%), S. arlettae (3%), S. vitulenuse (3%), S. piscifermentans (3%), S. capitis subsp. urealyticus (3%), S. saprophyticus subsp. saprophyticus (6.1%), S. caprea (6.1%), S. hominis subsp. hominis (6.1%), S. pasteuri (6.1%), S. hominis subsp. novobiosepticus (9.1%), S. hemolyticus (15.2%) and S. lugdunensis (21.2%). From the above findings the study concluded that, the coagulase- negative staphylococci were most common species isolated from HD patients with bacteremia while coagulase positive were isolated at low rate. Further studies are required to validate the results of the present study.