Molecular Screening for Human Papilloma Virus in Cervical and Oral Epithelial Cells of Sudanese Women with Cervical Lesions

Abstract

This is a descriptive study conducted in El Nou Hospital (Screening Center for Cervical Cancer )during the period from December 2009 to August aghast 2010. The study aimed to find out if there is any relation between HPV16 and18 in cervical cancer and oral epithelial cells infection. HPV was investigated among 50 women of whom, 40 were patients with cervical lesions and 10 were clinically healthy (as control group). For each study subjects two specimens were taken (cervical cells and oral cells). The DNA was extracted from tissue samples then polymerase Chain Reaction (PCR) was applied to investigate the HPV. In this study, among 40 patients with cervical lesions, HPV was identified in sixteen (40%) of study subjects. Of the eight (20%) positive with HPV subtype 16, six (15%) were identified with HPV subtype 18, and two (5%) were detected with both HPV subtypes 16 and 18. All of the clinically healthy cases were found negative. However, in specimen from oral cavity only one (2.5%) case was found positive for HPV subtype 16. HPV plays a critical role in the development of cervical cancer, in the Sudan. No clear relation between cervical and oral HPV infection. The study recommended screening more patients who suffer from cervical cancer using more advanced molecular techniques to confirm the role of the HPV in developing of cervical SCC in Sudan.