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Molecular Detection of Extended- spectrum Beta-Lactamase Genes in Enterobacter sakazakii Isolated from Clinical Specimens

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dc.contributor.author Abd El Razig, Weam Tag Elsir
dc.contributor.author Supervisor,- Humodi Ahmed Saeed
dc.date.accessioned 2013-11-26T10:02:42Z
dc.date.available 2013-11-26T10:02:42Z
dc.date.issued 2010-01-01
dc.identifier.citation Abd El Razig,Weam Tag Elsir.Molecular Detection of Extended- spectrum Beta-Lactamase Genes in Enterobacter sakazakii Isolated from Clinical Specimens/Weam Tag Elsir Abd El Razig;Humodi Ahmed Saeed.-Khartoum:Sudan University of Science and Technology,college of Medical Laboratory Science,2010.-20p. : ill. ; 28cm.-M.Sc. en_US
dc.identifier.uri http://repository.sustech.edu/handle/123456789/2480
dc.description Thesis en_US
dc.description.abstract This study was carried out in Research Laboratory of Sudan University of Science and Technology during the period between December 2009 to May 2010, to detect extended spectrum beta-lactamase genes in Enterobacter sakazakii Six bacterial strains were obtained from Research Lab. DNA was extracted from each strain using boiling method. Purity of DNA was conducted by run the extract on 1.5% agarose gel. Multiplex PCR technique was adopted to detect bla TEM, bla SHV and bla CTXM genes. This study showed that 6 strains (100%) of Enterobacter sakazakii were positive for bla TEM gene and the other genes were not detected. .The study concluded that TEM gene is responsible for ESPL in E. sakazakii strains en_US
dc.description.sponsorship Sudan University of Science and Technology en_US
dc.language.iso en en_US
dc.publisher Sudan University of Science and Technology en_US
dc.subject Spectrum Beta en_US
dc.title Molecular Detection of Extended- spectrum Beta-Lactamase Genes in Enterobacter sakazakii Isolated from Clinical Specimens en_US
dc.type Thesis en_US


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