Abstract:
This study was carried out in Research Laboratory of Sudan University of Science and
Technology during the period between December 2009 to May 2010, to detect extended
spectrum beta-lactamase genes in Enterobacter sakazakii
Six bacterial strains were obtained from Research Lab. DNA was extracted from each
strain using boiling method. Purity of DNA was conducted by run the extract on 1.5%
agarose gel. Multiplex PCR technique was adopted to detect bla TEM, bla SHV and bla
CTXM genes.
This study showed that 6 strains (100%) of Enterobacter sakazakii were positive for bla
TEM gene and the other genes were not detected.
.The study concluded that TEM gene is responsible for ESPL in E. sakazakii strains
