Detection of Cytomegalovirus Inclusion Bodies in Product of Conception Using Histochemical Staining Methods

Abstract

This is a retrospective hospital based study which conducted in Khartoum North teaching hospital, aimed to detect cytomegalovirus inclusion bodies in product of conception (aborted tissues) in chorionic villi by applying conventional histochemical stains, Haematoxyline and eosin, Machiavellos and PAS stain. Seventy six archived paraffin blocks from 2007 to 2010 of known cases of miscarriage at first trimester were collected, stained and examined for cytomegalovirus inclusion bodies. The patients’ information was collected from the archived short stay files. The patients’ age ranged between (22-47) year with mean age 31year. The histopathological results (H&E) reflect the following results‫׃‬The inclusion body represent13 (17.1%) haematoxyline and eosin in chorionic villi(trophoblast infected cell), 55(72.4%)normal chorionic villi ,6( 7.9%) degenerate chorionic villi,1(1.3%)choriocarcinoma and 1(1.3%)hydropic chorionic villi . Cytomegalovirus inclusion bodies were detected in 13(17.1%) by hamatoxyline and eosin stain, 3(3.9% )by Machiavello’s and 11(14.5%) by PAS stain. With significant relation between different three stains (p<0.05). According to the work the patients age suffering of miscarage ranged from 26-30 years ,the corelation between age and histological results was found to be statistcally insignificant( p>0.05). The study found that there is association between resident of patients and histopathological results,as most of patents from Bahri shemal (p<0.05). The study found no signficant relation between miscarriage number,clinical remarks and histopathologiacal results.(p>0.05). The study concluded that cytomegalovirus inclusion bodies were detected using histochemical staining method, haematoxyline and eosin, Machiavello stain and PAS .haematoxyline and eosin considered as abest method for detection of cytomegalovirus inclusion body, special stain applied as confirmatory tools.Cytomegallovirus infection appear as one of causes of miscarage.17.1%.