Abstract:
This is a retrospective hospital based study which conducted in
Khartoum North teaching hospital, aimed to detect cytomegalovirus
inclusion bodies in product of conception (aborted tissues) in chorionic villi
by applying conventional histochemical stains, Haematoxyline and eosin,
Machiavellos and PAS stain.
Seventy six archived paraffin blocks from 2007 to 2010 of known
cases of miscarriage at first trimester were collected, stained and examined
for cytomegalovirus inclusion bodies. The patients’ information was
collected from the archived short stay files.
The patients’ age ranged between (22-47) year with mean age 31year.
The histopathological results (H&E) reflect the following results׃The
inclusion body represent13 (17.1%) haematoxyline and eosin in chorionic
villi(trophoblast infected cell), 55(72.4%)normal chorionic villi ,6( 7.9%)
degenerate chorionic villi,1(1.3%)choriocarcinoma and 1(1.3%)hydropic
chorionic villi .
Cytomegalovirus inclusion bodies were detected in 13(17.1%) by
hamatoxyline and eosin stain, 3(3.9% )by Machiavello’s and 11(14.5%) by
PAS stain. With significant relation between different three stains (p<0.05).
According to the work the
patients age suffering of
miscarage
ranged from 26-30 years ,the corelation between age and histological results
was found to be statistcally insignificant( p>0.05).
The study found that there is association between resident of patients
and histopathological
results,as most of patents from Bahri shemal
(p<0.05).
The study found no signficant relation between miscarriage
number,clinical remarks and histopathologiacal results.(p>0.05).
The study concluded that cytomegalovirus inclusion bodies were
detected using histochemical staining method, haematoxyline and eosin,
Machiavello stain and PAS .haematoxyline and eosin considered
as abest
method for detection of cytomegalovirus inclusion body, special stain
applied as confirmatory tools.Cytomegallovirus infection appear as one of
causes of miscarage.17.1%.
