Evaluation of Reticulocyte Count using Manual Technique Versus Automated Technique in Healthy and Anemic Adults in Sudan

Abstract

This is a cross-sectional descriptive and analytical study, conducted at Omdurman Military Hospital during the period from January 2011 to July 2011. The aim of this study was to evaluate the reticulocyte count using manual versus automated technique in healthy and known anemic patients. Who attended to the Hospital. 100 Sudanese were informed about the study and agreed for participation was obtained. The healthy participants were 26%.and the anemic patients were 74%. 2.5 ml venous blood sample were collected in EDTA containers and investigate for reticulocyte count by manual technique and automated analyzer (sysmex XT2000i) Other tests Hb, PCV, RBCs count, MCV, MCH, and MCHC are obtained from the hospital record as complemenrty tests for evaluation of RBCs parameters The mean of reticulocyte count percentage using manual technique was (1.3%) and with the automated technique was (1.5%). There was no significant difference in the reticulocyte count in the two techniques .i.e. P value was (0.16) ‫‪Also reticulocyte in comparison between male and female‬‬ ‫‪participant, the mean of reticulocyte count percentage in male‬‬ ‫‪was (3.3%) and in female was (2.9%).there was no significant‬‬ ‫.)86.0‪deferent in reticulocyte count in both gender. i.e. ( P value‬‬ ‫:‪Conclusion‬‬ ‫‪It was found that the result of reticulocyte count does not‬‬ ‫‪affected by method used in count and also not affected by‬‬ ‫.‪gender‬‬