Estimation of some Haemostatic Parameters Among Sudanese Diabetes Mellitus Type 2 Patients in Khartoum state

Abstract

This is a descriptive cross sectional study which was conducted in Khartoum state during the period from April/ 2013 to July / 2013 to evaluate the effect of type 2 diabetes on coagulation tests (Prothrombin time, Activated partial thrompoblastin time, Thrombin time and Fibrinogen level). A 100 sample were collected from type 2 diabetic patient according to inclusion criteria and considered as case, and 50 samples were collected from healthy individual and considered as control.4.5 ml of fresh venous blood where collected from diabetic patient , after filling the questionnaire, in plastic container containing 0.5ml of 3.2 % tri sodium citrate solution for anticoagulation . Then the contents of the container were mixed and centrifuged at 3000 round / min for 15 minutes for preparation of platelets poor plasma (ppp).the ppp were tested for the Prothrombin time, Activated partial thrompoblastin time, Thrombin time and fibrinogen level by using the coagulometer. The results were analyzed by independent T test the SPSS computer programme. The result of cases revealed that Prothrombin time =14.1seconds , Activated partial thrompoblastin time=31.6 seconds , ,Thrombin time=5.8 seconds and fibrinogen=572.5 mg/dl . The results of control group revealed that Prothrombin time=13.2 seconds .Activated partial thrompoblastin time =29.4 seconds, Thrombin time= 7.8 seconds and fibrinogen level= 274.0 mg/dl. Over all the results were showed significant increase in PT , PTT and Fibrinogen level when compared with control group with P value > 0.00 (This increase within normal range), and significant decreased in TT when compared with control group with P value > 0.00. no significant change noticed in the different sex in APTT,TT and Fibrinogen with P value >0.05 but there was significant change noticed in the different sex in PT with p value<0.05.no significant change noticed in the different age group in PTT ,TT and Fibrinogen with P value >0.05 - but there was significant change noticed in the different age group in PT with p value<0.05.