Abstract:
These research is focusing on Phytochemical and antimicrobial studies of
some flavonoids
from tow medicinal plants Tamarix nilotica and
Hyphaene thebacia uses in Sudanese ethnomedicine .
The study confirm the present of flavonoids in these species with high
concentration, these may be related to uses in traditional medicine .
The conventional chromatographic
techniques used for isolation and
purification flavonoids compounds. The flavonoids were isolated from
butanol fraction of Tamarix nilotica and ethyl acetate fraction of
Hyphaene thebacia by thin layer and column chromatography.
The spectroscopic data of isolated flavonoids served as a valuable tool
in determining their structures. The confirmation of the structure of
isolated flavonoid is achieved by the analysis of different spectral data
(UV-VIS, IR, MS,1H and13C NMR).
The different spectroscopic analysis led to identification of five
compounds: tow from Tamarix nilotica (a flavone and a dihydroflavonol)
and three from Hyphaene thebacia (tow as flavones and one flavonol).
Evaluation of the antimicrobial activities of different extracts and isolated
compounds was carried out by the disc diffusion method, measured by
the diameter of the zone of inhibition.
The results exhibited variable susceptibilities of microorganism for
different extracts and pure compound .A significant antibacterial and
antifungi activity was related to butanol extract from tamarix nilotic. The
other
extracts showed
moderate to weak
inhibition on fungi
(Aspergillus niger& Candidas albicans)
The pure compounds (I&II) isolated from butanol extract of tamarix
nilotica also revealed significant antibacterial
activity and antifungi
activity. Compound IV isolated from ethyl acetate extract of Hyphaene
thebaica revealed moderate inhibition on bacteria and fungi. This study
demonstrated that the butanol fraction of Tamarix nilotica is potential
candidate for antimicrobial activity and deserves further optimization.
Antioxidant assay was performed by TLC using DPPH.
A significant antioxidant activity was related to butanol fractions of
tamarix nilotica and ethyl acetate fraction of Hyphaene thebaica.
Summary of the compounds isolated from the leaves of Tamarix
Hyphaene thebaica fruits
