In Vitro Rooting and Acclimatization of Sorghum bicolor L.Cultivar wad Ahmed

Abstract

The research was conducted in the Laboratory of Plant Cell and Tissue Culture, Commission for Biotechnology and Genetic Engineering, National Center for Research, to evaluate in vitro rooting and acclimatization of sorghum bicolar plantlets cultivar wad Ahmed. Plantlets of in vitro maintained stock plantlets come from callus were used as explant material in all experiments. plantlets obtained from in vitro callus cultured on hormone free-medium supplemented with two levels ofBAP (0.5 and 1.0) mg/l in combinations with NAA to evaluated multiple shoot formation. The best result for multiple shoots formation (12.8 shoots/explant) was obtained when the plantlets explants were cultured on MS medium supplemented with BAP at 1.0 mg/l combinations with NAA at 1.0 mg/l. In vitro regenerated shoots were rooted on MS medium without or with two levels (1.0 and 2.0 mg/l) of NAA and two levels of sugar (20 and 30) g/l. The best results for rooting percentage (90 %) and number of roots (3.5) were obtained when regenerated shoots were cultured on MS basal medium supplemented with NAA at 2.0 mg/l and sugar 30 g/l. Rooted plants were hardened and 70% of them survived under greenhouse conditions.