INVEESTIGATION OF URIC ACID IN BLOOD SERUM BY RAMAN SPECTROMETER

Abstract

In this work uric acid of different blood serum was investigated using Raman spectroscopy as invasive technique. Different samples (15) from patients with renal failure in homo dialysis unit – Soba University Hospital were collected. The concentration of Uric Acid was measured with conventional technique, and then the blood serum samples stored less than 80 c ̊. Raman spectra of samples was obtained without any preparation using Raman spectrometer with diode laser as excitation source (λ = 532 nm, P = 40 mW) using software associated with the spectrometer system. The spectra were truncated and the peak intensities at the assigned wavenumber in cm-1 were determined. The result revels the presence of peaks from most of the samples at their position (in wavenumber) with slight shifts and it indicated the presence of Uric Acid compound and the peaks are 471.9, 582.1, 628.4, 785,997.9, 1041.3,1234.6, 1269.9, 1594.2, 1653.2 cm-1. From the result above were a linear relation between the measured concentration and the intensity of the peak at the each wavenumber from samples was found which leads to an indication of the applicability of this technique in quantitative analysis.