Abstract:
In this work uric acid of different blood serum was investigated using Raman
spectroscopy as invasive technique. Different samples (15) from patients with renal
failure in homo dialysis unit – Soba University Hospital were collected. The
concentration of Uric Acid was measured with conventional technique, and then
the blood serum samples stored less than 80 c ̊. Raman spectra of samples was
obtained without any preparation using Raman spectrometer with diode laser as
excitation source (λ = 532 nm, P = 40 mW) using software associated with the
spectrometer system. The spectra were truncated and the peak intensities at the
assigned wavenumber in cm-1 were determined.
The result revels the presence of peaks from most of the samples at their position
(in wavenumber) with slight shifts and it indicated the presence of Uric Acid
compound and the peaks are 471.9, 582.1, 628.4, 785,997.9, 1041.3,1234.6,
1269.9, 1594.2, 1653.2 cm-1.
From the result above were a linear relation between the measured concentration
and the intensity of the peak at the each wavenumber from samples was found
which leads to an indication of the applicability of this technique in quantitative
analysis.
