Abstract:
Phytochemical screening the leaves of Aristolochia
bracteodlata revealed the presence of flavonoids, tannins,
alkaloids and glycosides. Paper sheets eluted with 15% acetic
acid allowed isolation of compounds I and II from the leaves of
Aristolochia bracteodlata.The partial structures of these isolates
were deduced on the basis of their spectral data(UV,NMR and
MS).The ethanol extract of Aristolochia bracteodlata was evaluated
,in vitro, for antimicrobial activity against five standard human
pathogens(Gram positive: Staphylococcus aureus and Bacillus
subtilis; Gram negative : Escherichia coli and Pseudomonasa
aeruginosa and the fungus Candida albicans using the cup plate
agar diffusion bioassay . Different antimicrobial responses were
observed. The activity ranged from high to moderate. Since the
biological activity of flavonoids is well documented now, thebioactivity of these extract could possibly be due to their
flavonoid content .
Saudi Trigonella foenum seed oil was analyzed by GC-MS and
antimicrobial potency of the oil was evaluated. 26 components
were detected by GC-MS analysis being dominated by: z,z-9,12-
octadecadienoic acid(35.85%), 9,12,15-octadecatrienoic acid
methyl ester(19.66%),hexadecanoic acid methyl ester(13.29%)
and z-9-octadecenoic acid methyl ester (10.13%) .The
antimicrobial activity of the oil was evaluated via the diffusion
bioassay against five standard human pathogens.The oil showed
excellent activity against the bacterial strain Staphylococcus
aureus in the concentration range : 100-25mg/ml. It also
exhibited excellent activity against the yeast Candida albicans
at 100mg/ml. The oil showed activity against all test organism at
100 mg/ml.
Foeniculum vulgare seed oil was studied by GC-MS. The oilwas also assessed for antimicrobial activity. Thirty two
components were detected by GC-MS analysis. Main
constituents are: 9-octadecenoic acid methyl ester(48.54%),
9,12-octadecadienoic acid methyl ester(28.50%), γ-
terpinene(4.98%), and β-pinene(3.51%).The antibacterial
activity of the oil was evaluated via the diffusion assay against
five standard human pathogens. Foeniculum vulgae oil showed
excellent activity against Staphylococcus aureus in the
concentration range : 100-25mg/ml. It also exhibited significant
activity against the yeast Candida albicans at 100mg/ml. The
fixed oil of Saudi Coriandrum Sativum was extracted by
maceration and analyzed by Gas Chromatography - Mass
Spectrometry where 52 constituents were detected.The oil was
also evaluated for antimicrobial potency.
