Efficiency of Recombinant DNA Vaccine Against Hepatitis B Virus in Health Care Workers in Some Hospital in Khartoum State

Abstract

This is a quantitative, descriptive, cross sectional study was conducted during the period from September to December, 2014. The objective of study was to assess the efficiency of HBV vaccine in Health Care Workers in some hospitals in Khartoum State. A total of eighty nine Hepatitis B vaccinated participants (n=89) were enrolled in this study. The participants, were screened before taking the vaccine and have no history of infection by Hepatitis B or other jaundice, all participants were vaccinated with three doses. The period ranges from 6 month to 10 years after last dose. All samples were screened by Immune Chromatography Test (ICT) to detect HBsAg. The titer of Anti-Hepatitis B antibody was estimated using Enzyme Linked Immune Sorbent Assay (ELISA). The number of participants were28 (31.5%) males, and 61(68.5%) females. The positive anti-HBsAg females were 45, while the positive males to antiHBsAg were 21. Smokers positive to anti-HBsAg ware 8, smoker negative to anti-HBsAg were 3, 58 of no smoker gave positive result.20of no smoker negative result. The efficiency of the vaccine varied from one group to another. The participants ; were groped-according to age as follow. 20-30 years (54) effective (41),and not effective(13),31-40years 26, effective(18)and not effective(8),41-50years 4, effective(4)and not effective(0), 51-60 years 3,effective(2) and not effective(1), 61-70 years2 effective(1)and not effective(1). The participants were also grouped according to the last dose of taken as follows: 6-20 month positive anti-HBsAg 26, negative anti-HBsAg3, 2135month positive anti-HBsAg 12, negative anti-HBsAg7 , 36-50 month positive anti-HBsAg 9, negative anti-HBsAg6, 51-65 month positive antiHBsAg 7, negative anti-HBsAg1, 66-80 month positive anti-HBsAg 3, negative anti- HBsAg0,81-95 month positive anti-HBsAg 1, negative antiHBsAg2, 96-110 month positive anti-HBsAg 4, negative anti-HBsAg2, and 111-125 month positive anti-HBsAg 4, negative anti-HBsAg2. The efficiency of vaccine was observed 66(74.2%) and not effective in 23(25.8%). The study concluded HBV rDNA vaccine was found to offer good seroprotection and was highly immunogenic in Health Care Workers. Further studies are required to validate results of this present study.