Detection of Providencia rettgeri in Urine Specimens using Real-time Polymerase Chain Reaction Technique

Abstract

This study was carried out in Khartoum state during the period between May 2007 to March 2008 to detect Providencia rettgeri in patients with urinary tract infection. Forty- six specimens of urine were collected from different hospitals in Khartoum state. Bacterial DNA was extracted from each urine specimen by using phenol chloroform method, and then the real time PCR technique was adopted to detect P. rettgeri in these specimens. This study showed that the positive results were 4.3% and the negative results were 95.7%. The study concluded that the real-time PCR is a powerful technique for fast identification of Providencia rettgeri from urine specimens compared to the bacteriological culture and it has the potential to be used in a diagnostic microbiology .laboratory