Study of Photosensitization Process for Phenoxazone 9 Dye Using Coherent and Incoherent Light Sources

Abstract

The aim of this research was the study of Phenoxazone 9 dye photosensitization; By using coherent and non coherent light sources; The emission spectroscopy was used to investigate the production of singlet oxygen, which has therapeutic effect from this dye after irradiation. Five samples acetone were prepared using ethanol, propanol , toluene, and chloroform to dissolve Phenoxazone 9 . Three light sources were used to irradiate the mentioned samples. Firstly, blue light emitting diode (LED) (450 - 470 nm) with power of 60 μW. Secondly, He Ne laser (632.8 nm), with output power of 1 mW. Thirdly, green diode laser (532 nm) with output power of 4 mW. A spectrometer connected to PC was used to collect and record the emission spectra of the samples with different time. The results showed that the most efficient solvents to produce singlet oxygen were acetone and ethanol, which gave positive results with each light source used. Toluene and chloroform produced singlet oxygen when coherent light source (laser) was used, but propanol succeeded to produce single oxygen with He Ne laser only. The best light source to produce singlet oxygen from phenoxazone 9 in the solvents was He Ne laser, unlike green diode laser and LED, where the first failed with propanol and the second failed with propanol, toluene and chloroform. The highest production of singlet oxygen form phenoxazone 9 was found when it dissolved in acetone and irradiated by green diode laser with exposure time of four minutes where two emission peaks of singlet oxygen at 703 nm and 634 nm were recorded.