Measurement of Hematological Parameters of Newborns Umbilical Cord Blood in Khartoum and Ibrahim Malik Teaching Hospitals

Abstract

This is a cross sectional study that aimed to measure some hematological parameters of neonatal cord blood. This study was conducted in Khartoum and Ibrahim Malik teaching hospitals during a period of four months (from February to June 2011) .Total of 100 Blood samples were collected from the umbilical cord of the newborns babies into sample bottles containing EDTA anticoagulant adequate for 2.5 ml of blood. All samples were analyzed for different haematological parameters complete blood count and reticulocyte count by using an automatic multi-parameter blood cell counter (Sysmex KX-N21) for CBC, and special reticulocyte stain (Brilliant creysl blue) for reticulocyte count. The data entry and analysis was done on computer package SPSS (Statistical Packages of Social Sciences).The study showed that hemoglobin mean value of healthy newborn cord blood at birth was (15.0 ± 1.6 )g/dl with range of (11.8 -18.2 )g/dl Where as the mean of total WBCs count was (11.4±4.3)x10 9/l with range of (2.8- 20.0) x 109/l. The mean of RBCs was (4.43±0.48)x1012/l with range of (3.47- 5.39)x1012/l and Platelet count mean(239 ±60) x109/l with range of (119-359) x109/l. Hct mean was (47.8± 5.3)% with range of (37.2-58.4)%. MCV mean (107.7±6.8)fl with range of (94.1-121.3)fl, MCH mean (33.9±2.1)pg with range of (29.7-38.1)pg and MCHC mean (31.5±1.6) g/dl with rang of (28.3-34.7 )g/dl. Neutrophil mean( 55±8)% with range of (39-71)%,lymphocyte mean(40±8)% with range of (24-56)%, monocyte mean (3 ±3)with range of (0-9)%, eosinophil mean (3±2)% with range of (0-7)% and basophil mean (0.04±0.2)% with range of( 0-1%) . Reticulocyte count mean (4.9±4.3)% with range of (2.3-7.5)% and NRBCs mean was (4.3±5.2)/100WBCs with range of (0-14.7)/100WBCS.