Molecualar Detection of Staphylococcus aureus Interotoxin (B) in Milk and Milk Products Using Polymerase Chain Reaction

Abstract

The aim of the study was to detect presence of Staphylococcus aureus and further investigate S. aureus isolates for enterotoxin B gene in milk and milk products During period from April to August 2012. A total of 150 milk and milk products (cheese, yoghurt samples) in Khartoum state were collected, all specimens were investigated using standard cultural and molecular methods. 18(12%) S. aureus were isolated from the collected specimens, among them 8 (44.4%) were enterotoxin B producers. The S. aureus isolates were recovered from the specimens as follows: 13 (72.2%) were isolated from cheese, 5 (27.8%) from milk, and non from yoghurt.