Frequency of Methicillin Resistant Staphylococcus aureus (MRSA) Nasal carriage and detection of mec A gene among Medical and non- medical staff at Khartoum Teaching Hospital

Abstract

During the period from April to October 2012, two hundred participants from Khartoum Teaching Hospital (KTH), (medical and non-medical staff) were enrolled in this study to detect the presence of Methicillin Resistant Staphylococcus aureus (MRSA) nasal carriage and to detect Methicillin resistant gene (mecA), out of them 10(58.8%) were male and 7(41.1%) female, their age range from 21-66 years with mean 1.35. Two hundred nasal swabs were collected and investigated by standard cultural, and molecular methods, Standard Kirby Bauer method was used to detect methicillin resistant. Out of the total, S. aureus was isolated from 43(21.5%) medical staff only among them 17(39.5%) were methicillin resistant. Most of the isolates were from age group 21-36 years and more frequent in male {10(58.8%)}, there were insignificant relation between the sexes (p=.732), age (p=.060) with regard to the MRSA nasal carriage. Molecular characterization with PCR revealed that among total (17) isolates of MRSA only 2(11.7%) possess mecA gene.