Characterization and Identification of Antibiotic-Producing Bacteria from Soil in North Kordufan State.

Abstract

The discovery of antibiotic from microbial source has yielded impressive number of compounds over the past 50 years. This study was carried out during the period March 2011- June 2011, to isolate antibiotic producing-bacteria from soil. Soil samples were collected in sterile plastic bag, then cultured by pouring plate technique on nutrient agar enriched with soil extract. The overlay method was adopted to isolate the antibiotic producer(s). Activity of antibiotic was evaluated aganist standard strains and clinical bacterial isolates. The Result revealed that different promising bacteria were isolated. Of the broad spectrum producers, one isolate was choosen and subsequently identified as Bacillus subtilis. The product was found to be active against Staphylococcus aureus, Pseudomonas species, and Salmonella species. in 5% ‫‫‫‪glucose concentration. It is concluded that ‫-‪Sudanese soil teems with antibiotic‬‬ ‬‬‫.‪producing bacteria and further investigations are required to validate this result‬‬