Detection of-Treponema pallidum antibodies among HBsAg positive blood donors using Enzyme Linked Immuno Sorbent Assay (ELISA) and Rapid Plasma Reagin (RPR) – Khartoum state

Abstract

This cross-sectional study was conducted in Khartoum, Sudan during the period from February to June 2015. Blood samples from136 hepatitis B positive blood donors with different ages (18-60) attending Khartoum Teaching Hospital and Ibn Seena hospital blood bank, were collected to detect seroprevalence of syphilis. Serum samples were tested for syphilis using Rapid plasma regain(RPR) and Enzyme Linked Immunosorbent Assay (ELISA) Anti –Treponemal antibodies were detected in all samples (100%) using RPR, and in 6 ( 4.4%) using ELISA , higher prevalence 3 (2.3%) were detected in age group 34-41year in age groups (18-25, 26-33,42-49 year) except above than 50 syphilis prevalence was detected in 0.7% in each group. According to marital status, positive results were obtained in married 4 (2.9% ) and in unmarried 2 (1.5%). All donors have no history of previous blood transfusion