Study of Cytomorphological Pattern of Cervical Cells and Sperm among Infertile Couples in River Nile State

Abstract

This study was conducted in Elddamer, Atbra and Barbar hospitals in the River Nile State, during the period from Decemper 2009 to May 2010. This study aimed at detecting the cytomorphological changes of infertile couples using cytological techniques. ٢00 samples were collected randomly from couples, distributed as follow, 70 cervical smears from infertile wives and 30 cervical smears from fertile wives as control, their age ranged from 18 to 45 years, 70 seminal smears from infertile husbands and 30 seminal smears from fertile husbands as control their age ranged from 18 to 66 years. The smears were prepared, fixed and stained using Papanicolaou technique then examined under the light microscope. The cytological assessment of the study group revealed the following findings. Normal cervical smear were found in 4(5.7%) samples, 28(40%) had bacterial infection, 9(12.9%) had monilia infection, 16(22.9%) had inflammatory cells, 6(8.6%) showed atypical cells, 5(7.1%) had human papilloma virus, 2(2.9%) had herpes simplex virus. In husbands semen smears; normal semen smear found in 27(38.6%), 22(31.4%) had bacterial infection, 21(30%) had inflammatory cells, 36(51.4%) had abnormal sperm morphology and 34(48.6%) had normal sperm morphology with significant relation between infertility and cytological changes (P.value = 0.000). In this study no significant relation between cytological changes and type of infertility, duration of infertility and contraceptive usage was found (P.value > 0.05). In this study asignificant relation between ages of infertile husbands and the cytological changes was found (P.value < 0.05). As acnclclusion of this study, the cytological changes in infertile couples were associated with infertility, and no relation between these changes and the type and the duration of infertility and contraceptive usage.